Ibicuruzwa

Reagent nshya ya Trinder nimbuto zikomoka kumazi aniline zikoreshwa cyane mubisubizo byo gusuzuma no gupima ibinyabuzima.Hariho ibyiza byinshi kurenza reagent ya chromogenic muburyo bwo kugena amabara ya hydrogène peroxide.Reagent nshya ya Trinder irahagaze bihagije kugirango ikoreshwe haba mubisubizo hamwe na sisitemu yo kugerageza imiyoboro.Imbere ya hydrogène peroxide na peroxidase, reagent ya Trinder yerekanwe ko yakira 4- aminoantipyrine (4-AA) cyangwa 3- methylbenzothiazole sulfonehydrazone (MBTH) mugihe cyo guhuza okiside.Ifite ibara rihamye cyane cyangwa irangi ry'ubururu.Kwinjiza amarangi y'irangi bifatanije na MBTH byikubye inshuro 1.5- 2 ugereranije n'irangi rifatanije na 4-AA;icyakora, igisubizo cya 4-AA cyari gihamye kuruta igisubizo cya MBTH.Substrate ihindurwamo okiside na okiside kugirango itange hydrogen peroxide.Hydrogene peroxide yibanze ihuye na substrate yibanze.Kubwibyo, ingano ya substrate irashobora kugenwa niterambere ryamabara ya okiside ihuza reaction.Glucose, inzoga, acyl-CoA na cholesterol birashobora gukoreshwa mugutahura izo substrate zifatanije nigitabo cyitwa Trinder reagent na 4-AA.Hano hari reagent 10 nshya ya Trinder irahari.Muri reagent nshya ya Trinder, TOOS niyo ikoreshwa cyane.Ariko, kubutaka bwihariye, kugerageza ibyiciro bitandukanye bya reagent ya Trinder birakenewe kugirango utezimbere uburyo bwiza bwo kumenya.