Igitabo g-glucosidase gene, bgl1G5, yakuwe muri Phialophora sp.G5 kandi byerekanwe neza muri pasitori ya Pichia.Isesengura ryikurikiranya ryerekanye ko gene igizwe na 1,431-bp ikarito yo gusoma ikubiyemo poroteyine ya acide amine 476.Urutonde rwa aminide acide ya bgl1G5 yerekanaga indangamuntu yo hejuru ya 85% hamwe na β-glucosidase iranga Humicola grisea yo mumuryango wa hydrocase ya glycoside 1. Ugereranije nabandi bagenzi babo, Bgl1G5 yerekanye ibikorwa byiza kuri pH 6.0 na 50 ° C kandi byari bihamye kuri pH 5.0–9.0.Byongeye kandi, Bgl1G5 yerekanye ubushyuhe bwiza kuri 50 ° C (6 h igice cyubuzima) hamwe nibikorwa byihariye (54.9 U mg - 1).Indangagaciro za K m na V zerekeza kuri p-nitrophenyl β-D-glucopyranoside (pNPG) yari 0.33 mM na 103.1 μ mol min - 1 mg - 1.Ubushakashatsi bwihariye bwerekana ko Bgl1G5 yakoraga cyane kurwanya pNPG, ifite intege nke kuri p-nitrophenyl β-D-selobioside (pNPC) na p-nitrophenyl-β-D-galactopyranoside (ONPG), kandi nta gikorwa na kimwe yari afite kuri selobiose.
