Ibicuruzwa

Ingaruka zo kumesa kubikorwa bya P-glycoprotein atpase: itandukaniro muguhagarika ibikorwa byibanze na verapamil.

P.Twifashishije imitsi ya membrane yateguwe kuva kumurongo wa selile irwanya DC-3F / ADX, twize kubyerekeranye no kwangirika kwa basal (ni ukuvuga mugihe hatabayeho ibiyobyabwenge) hamwe nibikorwa bya P-gp biterwa na verapamil biterwa na deterges zitandukanye, mugihe kidashubije, kimwe no kuri solubilizing, kwibanda.Iterambere rya membrane solubilisation hamwe no kwiyongera kwa detergent byakurikiranwe no gukwirakwiza urumuri hamwe nubushakashatsi bwa centrifugation.Kubidashiramo ingufu za detergent, ibikoresho byose byapimwe usibye DOC byatumye habaho guhagarika igice cyibikorwa bya P-gp ATPase, bitajyanye numubare wimyenda itandukanye yapimwe yashyizwe muri membrane.Isesengura ryakozwe na verapamil iterwa na P-gp igaragaza ko ibikorwa bya P-gp ATPase bihindurwa muburyo butandukanye nogukoresha ibintu bitandukanye kumashanyarazi.Rero, imikoranire yihariye hagati ya P-gp na detergents birashoboka cyane kuba aho kuba isi yose.Nyuma yo gukemurwa nubushakashatsi butandukanye bwapimwe, ibikorwa byibanze bya P-gp ATPase byahagaritswe rwose, usibye ko hariho CHAPS yashoboye kubungabunga iki gikorwa kurwego rwagereranywa nubupimye muri kavukire.Nyamara, gukora verapamil iterwa na P-gp ATPase yatakaye mugihe cya P-gp solubilisation na CHAPS, ariko yagaruwe nyuma yo kugabanuka kwa CHAPS munsi yibitekerezo bya micellar.Ibyo kureba byerekana imikoranire yihariye hagati ya P-gp na molekile ya CHAPS muri micelles ivanze.Muri rusange, amakuru yacu yerekana imikoranire yihariye P-gp / detergents ijyanye n’aho ibibanza bitwara ibiyobyabwenge biri kuri P-gp transembrane.