3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Umubare wa Cataloge | XD90075 |
izina RY'IGICURUZWA | 3 - [(3-Cholanidopropyl) dimethylammonio] -1-propanesulfonate |
URUBANZA | 75621-03-3 |
Inzira ya molekulari | C32H58N2O7S |
Uburemere bwa molekile | 614.88 |
Ibisobanuro birambuye | Ibidukikije |
Bihujwe Amategeko agenga ibiciro | 29242970 |
Kugaragaza ibicuruzwa
IR | Ugereranije no gukoreshwa |
Gutakaza Kuma | <2% |
TLC | Umwanya umwe |
Azote | 4.46 - 4.65 |
Kugaragara | Ifu yera |
Gukemura (10% muri H2O) | Birasobanutse kandi bitagira ibara |
Imikorere (0.1M muri H2O) | <10us / cm |
Suzuma HPLC | > 98% |
UV (5% muri H2O) A290nm | <0.5 |
UV (5% muri H2O) A400nm | <0.02 |
Ibirimo gukemura (by GC) Dimethylformamide | <2.0% |
3-
Igikorwa cyibinyabuzima: CHAPS, ikomoka kuri Cholicacide, ni ibikoresho bya zwitterionic bisohora poroteyine za membrane.CHAPS ikoreshwa muguhindura poroteyine-ADN zitandukanye kandi irashobora kugumana ibikorwa bya biohimiki ya poroteyine mugisubizo.
Gukoresha: CHAPS ni detwent zwitterionic detergent ya membrane biochemie.Irashobora gukoreshwa muguhindura poroteyine za membrane no guhagarika poroteyine-proteyine.CHAPS ifite uburemere buke bwa micellar molekulari (6.150) hamwe na micelle ikomeye cyane (6-10 mM) bityo rero irashobora gukurwa murugero na dialyse.Irakwiriye kandi guhinduranya poroteyine muri isoelectric yibanze hamwe na 2D electrophorei.CHAPS ikoreshwa muburyo bwa kavukire (urea-idafite) isoelectric yibanze kandi wasangaga itanga igisubizo cyiza kubikorwa bimwe na bimwe byateguwe na proteine ziterwa.Mubisanzwe kwibanda hagati ya 2-4% (w / v) bikoreshwa kuri isoelectric yibanda kuri geles.
Imikoreshereze: Zwitterionic detergent ya detergent ya membrane biochemie ya membrane, ishobora gukoreshwa muguhindura poroteyine za membrane no guhagarika poroteyine-proteyine;birakwiriye kandi gushiramo poroteyine muri isoelectric yibanze hamwe na electrophorei-ebyiri;bikunze gukoreshwa muri Non-denaturing (nta urea) kwibanda kuri isoelectric;Gukuramo RNA na ADN, nta gikorwa cya RNase na DNase
Imikoreshereze: Amphoteric surfactant, CHAPS ni detergent zwitterionic detergent, protein lysate yo gukuramo poroteyine ya membrane no guhuza imikoranire hagati ya poroteyine na proteyine.