Bagiteri yitwa entomopatogeneque, Xenorhabdus nematophila, itera ubudahangarwa bw'udukoko twibasiwe no guhagarika ibikorwa bya fosifolipase A (2) (PLA (2)).Vuba aha, gene yatewe na immunite ya PLA (2) yavuye mu nyenzi y'ifu itukura, Tribolium castaneum.Ubu bushakashatsi bwakoresheje iyi gene ya PLA (2) muri bagiteri yerekana imiterere ya bagiteri kugirango ikore enzyme ya recombinant.Recombinant T. castaneum PLA (2) (TcPLA (2)) yerekanye ibikorwa byayo biranga enzyme hamwe nubushyuhe bwa substrate, pH, nubushyuhe bwibidukikije.Ibiranga ibinyabuzima byahujwe nubwoko bwibanga bwa PLA (2) (sPLA (2)) kubera ko ibikorwa byayo byahagaritswe na dithiothreitol (igabanya umukozi wa disulfide) na bromophenacyl bromide (inhibitori ya sPLA (2)) ariko ntabwo byakozwe na methylarachidonyl fluorophosphonate (ubwoko bwa cytosolike bwihariye bwa PLA (2)).Umuco wa X. nematophila warimo PLA (2) ibuza ibintu (s), byari byinshi cyane mubitangazamakuru byabonetse mugihe cyo gukura kwa bagiteri.Ibintu bya PLA (2) byabujije ubushyuhe kandi byakuwe mu bice by’amazi n’ibinyabuzima.Ingaruka z'igice cya PLA (2) -kubuza gukingira immunosuppression ya T. castaneum cyagereranijwe kimwe nicyaturutse kubuza imvugo ya TcPLA (2) kwivanga kwa RNA.
