Ibicuruzwa

Cysteine ​​(Cys) igira uruhare runini mubikorwa byinshi byimikorere ya selile ya eukaryotic kandi kuyibona muri selile bifite akamaro kanini.Nyamara, glutathione (GSH), homocysteine, N-acetyl-L-cysteine ​​nizindi thiol zibuza cyane kumenya Cys.By'umwihariko, GSH ibangamira cyane kumenya Cys selile (30–200 μM) kubera ubwinshi bwayo bwo mu nda (1-10 mM).Muri iki gikorwa, herekanwa off-on fluorescent probe (HOTA) kugirango hamenyekane Cys.Iperereza rifite ibyiyumvo byiza cyane kandi bihitamo guhitamo Cys selile: hiyongereyeho 200 μM Cys, ubukana bwa fluorescence ya probe (10 μM) bwiyongereyeho inshuro 117 kandi igipimo cyo gutahura cyabazwe kuri 13.47 μM, kiri hasi kuruta selile ya Cys yibanze;iperereza ryanatoranijwe guhitamo 30–200 μM cysteine ​​hejuru ya 1-10 mM glutathione.Kubwibyo, ubushakashatsi bwerekana amashusho bwakorewe hamwe na HOTA.Byongeye kandi, ibisubizo bya thiol-blocki ng na synthesis ya GSH ibuza ubushakashatsi byemeje ko imyuka yo mu nda ituruka ahanini ku mikoranire hagati ya Cys na HOTA.