PIPES Cas: 5625-37-6 Ifu ya kristaline yera 99% ABTS DIAMMONIUM SALT ULTRA YIZA
| Umubare wa Cataloge | XD90117 |
| izina RY'IGICURUZWA | PIPES (Piperazine-1,4-bis (2-etanesulfonike aside)) |
| URUBANZA | 5625-37-6 |
| Inzira ya molekulari | C8H18N2O6S2 |
| Uburemere bwa molekile | 302.37 |
| Ibisobanuro birambuye | Ibidukikije |
| Amategeko agenga ibiciro | 2933599 |
Kugaragaza ibicuruzwa
| Ibyuma biremereye | <5ppm |
| Gutakaza Kuma | <1.0% |
| Gukemura | Igisubizo gisobanutse, kitagira ibara (5% 1N NaOH) |
| Suzuma | 99 - 101% |
| Kugaragara | Ifu ya kirisiti yera |
PIPES [piperazine-N, N′-bis (acide 2-etanesulfonique)] ikoreshwa kenshi nka bffer muri biochemie.Nibikoresho bya etanesulfonike acide yakozwe na Nziza nibindi.mu myaka ya za 1960.PIPES ifite pKa hafi ya physiologique pH ituma iba ingirakamaro mubikorwa byumuco.Byanditswe kugirango hagabanuke igihombo cya lipide mugihe uhinduranya amateka ya glutaraldehyde mumyanya y'ibimera ninyamaswa.Umukozi woherejwe na pKa hafi ya pH physiologique.
Antimicrobial peptide magainin 2 ikora imyenge mumyanya ya lipide kandi itera membrane kwinjira mubintu bigize selile.Nubwo iyi myuka ishobora kuba intandaro yibikorwa bya bagiteri, uburyo bwo gukora pore buracyasobanuka neza.Twakoze iperereza ku buryo burambuye imikoranire ya magainin 2 na lipide membrane dukoresheje imitsi nini ya unilamellar (GUVs).Guhambira magainin 2 kuri lipid membrane ya GUVs byongereye impinduka zagace mubice bya membrane, δ, byagereranyaga nubuso bwa magainin 2, X. Ibi byerekana ko igipimo gihoraho cya magainin 2-iterwa na bibiri -inzibacyuho ya leta kuva muri reta idahwitse kuri pore leta yiyongereye cyane hamwe no kwiyongera δ.Umuvuduko wa lipid membrane nyuma yo kwifuza kwa GUV nawo watangije magainin 2-iterwa na pore.Kugirango hamenyekane aho magainin 2 iherereye, imikoranire ya carboxyfluorescein (CF) -yanditseho magainin 2 (CF-magainin 2) hamwe na GUV imwe irimo iperereza rya fluorescent probe, AF647, hakozwe iperereza hakoreshejwe microscopi conocal.Mugihe habuze impagarara bitewe nicyifuzo, nyuma yimikoranire ya magainin 2 ubukana bwa fluorescence bwurugero rwa GUV bitewe na CF-magainin 2 bwiyongereye vuba kugeza ku giciro gihamye, cyagumye gihoraho igihe kirekire, kandi kuri 4-32 s mbere intangiriro yo kumeneka kwa AF647 ubukana bwa rim bwatangiye kwiyongera byihuse kurindi gaciro rihamye.Ibinyuranye, imbere yubushyamirane, nta kwiyongera kwimbaraga za rim mbere yuko itangira kumeneka ryagaragaye.Ibisubizo byerekana ko magainin 2 idashobora guhinduranya kuva hanze ikerekeza monolayeri y'imbere kugeza mbere yuko habaho pore.Dufatiye kuri ibyo bisubizo, twanzuye ko pore ya magainin 2-iterwa na pore ni irambuye ikora kandi irambuye monolayeri y'imbere ni imbaraga nyamukuru itera ibibyimba.
