URUBUGA RWA ONPG: 369-07-3 98.0% Min Yera Kuri Off-Ifu yera
| Umubare wa Cataloge | XD90006 |
| URUBANZA | 369-07-3 |
| izina RY'IGICURUZWA | ONPG (2-Nitrophenyl-beta-D-galactopyranoside) |
| Inzira ya molekulari | C12H15NO8 |
| Uburemere bwa molekile | 301.25 |
| Ibisobanuro birambuye | 2 kugeza 8 ° C. |
| Amategeko agenga ibiciro | 29400000 |
Kugaragaza ibicuruzwa
| Isuku (HPLC) | Min.98.0% |
| Kugaragara | Umweru kugeza kuri -ifu yera |
| Igisubizo(1% mumazi) | Birasobanutse, bitagira ibara kugeza igisubizo cyumuhondo |
| Ibirimo Amazi(Karl Fisher) | Icyiza.0.5% |
| Guhinduranya optique yihariye [α] D20 (c = 1, H2O) | - 65.0 ° C kugeza kuri -73.0 ° C. |
Ikiganiro ku kizamini cya ONPG (test-galactosidase ikizamini)
Ibibazo byagaragaye kenshi vuba aha: 1. Kuki ikizamini cya ONPG cyakoreshwa mugutandukanya fermentation ya lactose yatinze?2. Kuki kuki igihugu gisanzwe kivuga ko ari ngombwa gukoresha 3% sodium chloride trisaccharide fer (cyangwa icyuma cya trisaccharide) mugupimisha ONPG?3. Kuri Vibrio parahaemolyticus, mugihe ukora ikizamini cya OPNG, kuki toluene igomba kongerwaho ibitonyanga ukurikije ibipimo?Ni uwuhe murimo?
Isosiyete yacu yasuzumye amakuru menshi, ayivuga muri make, kandi ayasangiza nawe hepfo:
Ihame: Izina ryigishinwa rya ONPG ni o-nitrobenzene-β-D-galactopyranoside.ONPG irashobora guhindurwamo hydrolyz muri galactose n'umuhondo o-nitropenol (ONP) na β-galactosidase, bityo ibikorwa bya β-galactosidase birashobora gutahurwa nihinduka ryibara ryumuco.
Lactose ni isukari mikorobe nyinshi ikeneye kumenya.Metabolism yayo isaba enzymes ebyiri, imwe ni selile permease, lactose yinjira muri selile munsi ya permease;ikindi ni β-galactosidase, hydrolyzes lactose muri galaktose.Lactose na glucose.β-Galactosidase irashobora kandi gukora kuri ONPG kugirango hydrolyze ibe galaktose n'umuhondo o-nitropenol (ONP).Irashobora gukorwa muri 24h, ndetse na lactose yatinze fermenters.Kubwibyo, isobanura ibyavuye mu kwitegereza gutoranya umuco 1 uhereye kuri agar no kuyitera mu ruziga rwuzuye kuri 36 ° C kuri 1-3h na 24h.Niba β-galactosidase ikozwe, izahinduka umuhondo muri 1-3h, niba nta enzyme nkiyi, ntabwo izahindura ibara muri 24h.
Ukurikije imisemburo ibiri yavuzwe haruguru, ibinyabuzima bishobora kugabanywamo ibyiciro bikurikira:
1 lactose-fermenting (amasaha 18-24) bagiteri zifite permease na gal-galactosidase P + G +;
2 Gutinda kwa fermenter ya lactose (gufata amasaha arenze 24) kubura permease ariko ufite galactosidase: P- G +.
3 Fermenters idafite lactose idafite permease na galactosidase: P- G-.
Ikizamini cya ONPG kirashobora gukoreshwa mu gutandukanya bagiteri ya lactose-lag-fermenting (P-G +) na bagiteri ya lactose idasembuye (PG-), nka:
1 Tandukanya fermenters yatinze (P- G +) na fermenters itari lactose (P- G-).
(a) Citrobacter (+) na Salmonella arizonae (+) wo muri Salmonella (-).
(b) Escherichia coli (+) kuva Shigella sonnei (-).
Kuki ubushakashatsi bwa ONPG bwakozwe hakoreshejwe umuco wijoro kuri 3% sodium chloride ferric trisaccharide (fer trisaccharide)?Isosiyete yacu yagishije inama amakuru menshi, ariko nta magambo asobanutse.Gusa kurubuga rwa FDA, handitswe ngo "Shyira imico kugirango isuzumwe inshuro eshatu isukari fer agar slants hanyuma ikore kuri 18 h kuri 37 ° C (cyangwa ubundi bushyuhe bukwiye, nibisabwa). Intungamubiri (cyangwa izindi) za agar zirimo 1.0 % lactose irashobora kandi gukoreshwa. "bisobanura: bagiteri yipimishije yatewe mu cyuma cya trisaccharide kandi ikagira umuco kuri 37 ° C kuri 18h.Intungamubiri za agar slants (cyangwa izindi) zikoreshwa zirimo 1% lactose nazo ziremewe.Kubwibyo, hafatwa umwanzuro ko icyuma cya trisaccharide kirimo lactose.Nyuma yo gukura nijoro, bagiteri zitanga umusaruro β-galactosidase.Ukoresheje za bagiteri, ONPG irashobora kubora na gal-galactosidase byihuse.Ikintu cyo kugerageza kirihuta kandi kigaragara neza.Mubyongeyeho, ibitonyanga byongeweho toluene hamwe nogeswa amazi kuminota 5 byose murwego rwo kurekura byuzuye β-galactosidase.
