Ibicuruzwa

Imiterere ya fiziki ya chimique Umuti wa acridine hamwe numunyu wacyo byerekana fluorescence cyangwa icyatsi kibisi.Gukoresha ibisubizo byumunyu bifite fluorescence yicyatsi, kandi iyo byongeye kuvangwa, kubera hydrolysis yumunyu, bihinduka acridine yubusa, yerekana fluorescence yumutuku.Igisubizo cyamazi ni alkaline nkeya kandi ikora hamwe na acide organic organique ikora umunyu.Acridine irahagaze neza, imiterere yayo isa na anthracene, kandi imiterere yimiti nayo irasa cyane.Umwuka hamwe nigisubizo byombi birakaze, bikarakaza cyane uruhu nibibondo, kandi guhumeka umwuka bishobora gutera inkorora.

Nka probe ya luminescent, ikoreshwa mukwiga chip ya gene.Igisubizo cyanditseho acridan (9,10-dihydroacridine) nka substrate na fosifata ya alkaline, itanga chemiluminescence ikabije.Itanga ibyiyumvo byikirenga kandi byoroshye gukoresha ikoreshwa rya alkaline fosifata mugihe cyo gutahura chemiluminescent.Fosifata ya alkaline iboneka mugihe kitarenze 10-19 mol, impinga byihuse kugirango igabanye igihe cyo gutahura no kongera ibicuruzwa, kandi umusozi wumurongo wa kalibrasi uteganijwe hamwe na logarithmic ingana na 1.0.Ingano imwe cyangwa nyinshi ya enzyme itanga ingano imwe cyangwa myinshi ya luminescence, luminescence ikomeza - ntibisaba cyane mugihe cyo gusuzuma.Ubukomezi bwa Luminescence burashobora gusomwa uhereye kumurongo wa kalibrasi yakozwe mugihe icyo aricyo cyose, kandi ibisubizo byisesengura ntabwo byumva ubushyuhe buri hagati ya 22 ° C - 35 ° C, bikagabanya ukuri gukenewe kugirango ubushyuhe bugabanuke.

Gushyira mu bikorwa: Irashobora gukoreshwa mubirango bya poroteyine, antigene, antibodies, acide nucleic (ADN, RNA), nibindi.