Ibicuruzwa

Uburyo bukomeye bwo gukingira immunoassay yo kumenya progesterone muri plasma yabantu byasobanuwe, bifashisha chemiluminescence nkibihe byanyuma.Projesterone-isoluminol conjugate ikora nk'ikimenyetso cya chemiluminescent.Igice cya IgG cya antiserum kuri progesterone-11 alpha-hemisuccinate bovine serumu albumin yandikirwa gusa kurukuta rwa Lumacuvettes P polystyrene yipimisha.Nyuma yo guhuza reaction, cuvettes zogejwe.Umusaruro woroheje wa conjugate uhujwe na okiside hamwe na sisitemu ya H2O2-microperoxidase kuri pH 13 wagabanutse hamwe no kwiyongera kwa progesterone yubusa muburyo bwa log-umurongo hejuru ya 15-1000 pg / tube.Immunoassay ya chemiluminescence igereranywa na radioimmunoassay kubijyanye na sensibilité, umwihariko, neza kandi neza.Isuzuma ritanga ibyiza byihuta no koroshya kwikora.Mubyongeyeho, ubu buryo ntabwo bukubiyemo gukoresha radioisotopi cyangwa intambwe ya centrifugation.