Icyatsi kibisi SF Cas: 5141-20-8 Ifu yijimye
| Umubare wa Cataloge | XD90538 |
| izina RY'IGICURUZWA | Icyatsi kibisi SF |
| URUBANZA | 5141-20-8 |
| Inzira ya molekulari | C₃₇H₃₄N₂Na₂O₉S₃ |
| Uburemere bwa molekile | 792.86 |
| Ibisobanuro birambuye | -15 kugeza kuri 20 ° C. |
| Amategeko agenga ibiciro | 32129000 |
Kugaragaza ibicuruzwa
| Kugaragara | Ifu yijimye |
| Suzuma | 99% |
| Gukemura | Gushonga mumazi kugirango utange igisubizo kibisi |
Kugirango usuzume gahunda iranga umutekano hamwe n’umutekano w’amabara mashya ashobora kubagwa mu nda. Amabara atandatu yashyizwe mu iperereza: icyatsi kibisi SF (LGSF) cyumuhondo, E68, ubururu bwa bromophenol (BPB), ubururu bwa Chicago (CB), rhodamine 6G, rhodulinblau -ibanze 3 (RDB-B3).Amabara yose yashongeshejwe hanyuma akayungurura mumuti wa saline iringaniye.Ibintu bikurura urumuri rwa buri rangi byapimwe ku gipimo cya 0,05% hagati ya 200 na 1000 nm.Ibiranga irangi byasuzumwe no gusiga lens capsule tissue na epiretinal membrane (ERMs), bivanwaho bidakorewe, hamwe n’ibara ry’amabara ya 1.0%, 0.5%, 0.2%, na 0.05%.Amaso ya porcine yuzuye (igihe cya postmortem, amasaha 9) nayo yaranduye.Uburozi bujyanye n'irangi bwasuzumwe n'ikizamini cya colimetric (MTT) gipima kubuza kwanduza ingirabuzimafatizo epitelium (RPE) ikwirakwizwa ry'uturemangingo (ARPE-19 na selile y'ibanze ya RPE, ibice 3-6).Ubuzima bwakagari nabwo bwapimwe hashingiwe ku mabara abiri ya fluorescence selile-viability assay.Irangi ryakozweho ubushakashatsi ku gipimo cya 0.2% na 0,02% .Amabara yose yakozweho ubushakashatsi muri ubu bushakashatsi yandujije capsules z'umuntu, yakuweho mu buryo budasanzwe;ERMs, yashonje mugihe cyo kubaga macular pucker;n'amaso ya porcine amaso, bitewe nubushakashatsi bwakoreshejwe.Uburebure burebure bwumurongo ntarengwa wamabara yari murwego rwa 527 kugeza 655 nm kuri concentration ya 0.05%.Rhodamine G6 na RDB-B3 bagaragaje ingaruka mbi ku ikwirakwizwa rya selile ARPE-19 ku gipimo cya 0.2% kandi ntibakurikiranwa n’iperereza ryakozwe mu tugari twibanze twa RPE.Amabara ane asigaye yerekanaga ko nta ngaruka z'uburozi kuri ARPE-19 no gukwirakwiza selile ya RPE yibanze kuri 0.2% na 0.02%.Ubuzima bwakagari bwatewe na LGSF yumuhondo (0.2%) na CB (0.2% na 0.02%).Amabara abiri (E68 na BPB) yerekanaga ko nta burozi bufite muri vitro.Isuzuma rifatika ryerekana amarangi yo gukoresha imitsi itagaragara ni itegeko.Muri ubu bushakashatsi hagaragaye amarangi ane afite ibimenyetso biranga irangi, hamwe muri ayo marangi abiri nta ngaruka zifite ubumara bugaragara ku ngirabuzimafatizo za RPE muri vitro.
