Ibicuruzwa

Infrared spectroscopy yakoreshejwe mugushushanya imikoranire ya substrate-protein: impinduka zijyanye na sarcoplasmic reticulum Ca (2 +) - ATPase kuri nucleotide ihuza na fosifora ya ATPase yakurikiranwe hakoreshejwe insimburangingo ya ATP na ATP (2'-deoxy-ATP, 3 '-deoxy-ATP, na inosine 5'-triphosifate), zahinduwe mumatsinda yihariye yimikorere ya substrate.Guhindura kuri 2'-OH, 3'-OH, hamwe nitsinda rya amino rya adenine bigabanya urugero rwo guhuza-guhuza ihinduka rya ATPase, hamwe ningaruka zikomeye zagaragaye kuri ebyiri zanyuma.Ibi birerekana ibyiyumvo byimiterere ya nucleotide-ATPase kumikoranire yabantu hagati ya nucleotide na ATPase.Amatsinda yose yize ni ngombwa muguhuza no guhuza itsinda ryatanzwe na ligand hamwe na ATPase biterwa nimikoranire yandi matsinda ya ligand.Fosifora ya ATPase yagaragaye kuri ITP na 2'-deoxy-ATP, ariko ntabwo kuri 3'-deoxy-ATP.Nta sano ihari iri hagati yurwego rwimpinduka zijyanye no guhuza nucleotide nigipimo cya fosifora yerekana ko urugero rwuzuye rwimpinduka ziterwa na ATP rutari itegeko kuri fosifora.Nkuko byagaragaye kuri nucleotide-ATPase, ihinduka rya mbere rya fosifori ya ATPase hagati ya E1PCa (2) naryo riterwa na nucleotide, byerekana ko leta za ATPase zifite ihinduka rito ugereranije nuko byari byitezwe mbere.