Ibicuruzwa

Adenosine ni endogenous igenga amajwi y'amaraso.Iki gikorwa cya adenosine kirangizwa no gufata no guhindagurika kwa selile na microcascular endothelial selile (MVEC).Abatwara ubwiganze bwiganjemo uruhare ni ENT1 (nucleoside ya nucleoside itwara abantu 1).MVEC iragaragaza kandi transport ya nucleobase (ENBT1) igira uruhare mukuzunguruka kwa selile ya metabolite ya adenosine nka hypoxanthine.Impinduka muri bumwe muri ubwo buryo bwo gutwara bwagira ingaruka kuri bioactivite ya adenosine na metabolism yayo, harimo no gukora radicals yubusa.MVEC yitandukanije n'imitsi ya skeletale ya ENT1 (+ / +) na ENT1 (- / -) imbeba zatewe na okiside iterwa na ischemia yigana / reperfusion cyangwa menadione.Ibikorwa byimikorere ya ENT1 na ENBT1 byasuzumwe hashingiwe kuri zero-trans flux kinetics ya radiolabel substrates.Habayeho kugabanuka k'umuvuduko wa hypoxanthine wunganirwa na ENBT1 na ENT1 (+ / +) MVEC yavuwe na menadione cyangwa nyuma yo guhura nibibazo bigereranya isc hemia / reperfusion.Muri ibyo bihe byombi, superoxide isenya mimetic MnTMPyP yerekanaga igihombo cyibikorwa bya ENBT1, bikagira uruhare runini rwa superoxide mubisubizo.Ibinyuranye, MVEC yitandukanije nimbeba za ENT1 (- / -) yerekanaga ko itagabanije ibikorwa bya ENBT1 nyuma yo kuvurwa na menadione cyangwa ischemia yigana / reperfusion, ariko bari bafite urwego rwo hejuru rwibikorwa bya catalase ugereranije na ENT1 (+ / +) MVEC.Aya makuru yerekana ko ibikorwa bya ENBT1 byagabanutse muri MVEC hasubijwe kwiyongera kwa radical ya superoxide ifitanye isano no gukomeretsa ischemia / reperfusion.MVEC yitandukanije nimbeba ya ENT1 (- / -) ntabwo yerekana uku kugabanuka muri ENBT1, birashoboka bitewe nibikorwa byiyongera bya catalase.