Ibicuruzwa

1.Metabolite yerekana uburyo nibikoresho byingenzi byo gupima ibizenga bya metabolite muri sisitemu y'ibinyabuzima.Mugihe uburyo bwinshi bwo gushushanya metabolite bwerekana ubukana bugereranije cyangwa biterwa nuburinganire bwimbere bugereranya metabolite zose, icyifuzo cyibanze kugirango umuntu asobanure umubare wa pisine ya metabolite muri selile biologique na fluide ni ukugena ibitekerezo byuzuye.Dutangariza hano hejuru-byinjira cyane kandi byoroshye gazi chromatografiya / tandem mass spectrometrie (GC / MS / MS) igamije uburyo bwa metabolite profiling uburyo butuma umubare wuzuye wa metabolite wamenyekanye.Uburyo bushingiye kuri methyl chloroformate derivatisation no kugereranya ukoresheje spiking ingero hamwe na metero ya metabolite itandukanye ikomoka hamwe na reagent de derivatisation.Ingaruka ya elegitoroniki gakondo isimburwa na ionisiyonike yimiti kuva iyanyuma kugeza murwego runini cyane ibika ion ya molekuline nibindi bice biremereye bya molekile.Ibi byatumye ea sier guhitamo inzibacyuho zidasanzwe za MS / MS muri metabolite nyinshi.Kugeza ubu, uburyo bushya bwa GC / MS / MS bugizwe na metabolite 67 isanzwe yibanze muri yo inyinshi muri zo zikaba ziri mu matsinda ya acide organic amine na nonamino.Twerekana uburyo bukoreshwa muburyo bwinkari na serumu.Uburyo ni iterambere ryibanze ryuburyo bugezweho bwa GC / MS metabolite yerekana ishusho ya aside amine na acide organic nonamino.

2.24S-Hydroxycholesterol (24OHC) na 27-hydroxycholesterol (27OHC) ni oxysterol ebyiri zubatswe mu buryo butandukanye - izambere zakozwe mu bwonko ndetse nizindi zakozwe ku rugero ruto mu bwonko kuruta izindi ngingo nyinshi.HYPOTHESIS YO GUKORESHWA: Kwangirika kwa neuronal na / cyangwa demyelination bitera kwiyongera kwa 24OHC kuva mubwonko bikinjira mumazi ya cerebrospinal fluid (CSF), mugihe inzitizi yamaraso yubwonko bwubwonko itera kwiyongera kwa 27OHC kuva mukuzenguruka muri CSF.Isotope dilution-mass spekrometrike yakoreshejwe mu gusuzuma oxysterol ebyiri muri CSF na plasma ku barwayi barenga 250 barwaye indwara zifata ubwonko na geriatricike.Urwego rwa CSF rwa oxysterol ebyiri rwibasiwe cyane n'indwara zitandukanye kuruta urwego rwa plasma.Abarwayi bafite indwara zifata demyelinine bariyongereyeho 24OHC muri CSF bafite igipimo cya 24OHC / 27OHC.Abarwayi barwaye meningite muri rusange bari bafite urugero rwinshi rwa steroid hamwe na 24OHC / 27OHC nkeya.Abarwayi barwaye Alzheimer bariyongereyeho gato 24OHC muri CSF hamwe no kwiyongera kwa 27OHC.Abarwayi bafite sclerose nyinshi bari bafite imyumvire yo kugira urwego rwo hejuru rwa 24OHC mugihe cyibikorwa bifite igipimo kinini cya 24OHC / 27OHC.Ibipimo bya oxysterol ebyiri muri CSF na plasma birashobora kwiyongera cyane muburyo bwa biohimiki busanzwe bwo gusuzuma indwara zifata ubwonko.