Ibicuruzwa

Hepatotoxicity y'abakandida ibiyobyabwenge nimwe mubibazo byingenzi mugupima ibiyobyabwenge mugihe cyo kuvumbura ibiyobyabwenge hakiri kare.Kumenya umwijima wa okiside ya hepatike birashobora kuba ikimenyetso cyambere cya hepatotoxicity kandi bigirira akamaro guhitamo ibiyobyabwenge.Glutathione (GSH) na glutathione disulfide (GSSG), nka kimwe mu bintu nyamukuru bigenga redox igenga abashakanye, bigira uruhare runini mu kurinda ingirabuzimafatizo ziterwa na okiside iterwa n'ubusumbane buri hagati ya porokiside na antioxydants.Kugena umubare wibipimo bya GSSG / GSH hamwe nubushuhe bwa GSH na GSSG byakoreshejwe kugirango berekane imbaraga za okiside mu ngirabuzimafatizo no mu ngingo.Muri ubu bushakashatsi, twagerageje uburyo bwo gukoresha igipimo cya biliary GSSG / GSH nka biomarker kugirango tugaragaze impagarara za okiside ya hepatike nuburozi bwibiyobyabwenge.Ibintu bine bizwiho guhindura urwego rwa GSH na GSSG byageragejwe muri ubu bushakashatsi.Diquat (diquat dibromide monohydrate) na acetaminofeni byahawe imbeba.Paraquat na tert-butyl hydroperoxide yatanzwe d ku mbeba kugirango habeho impinduka za biliary GSH na GSSG.Ikirangantego GSH na GSSG byapimwe hifashishijwe umurongo wa kalibrasi wateguwe hamwe na artile artificiel kugirango harebwe ingaruka zose za matrix mu isesengura rya LC-MS no kwirinda kwivanga kwa GSH na GSSG.Hamwe n'ingero enye (mu mbeba n'imbeba) zimpinduka zatewe nibiyobyabwenge muri kinetics ya biliary GSSG / GSH, ubu bushakashatsi bwerekanye ubushobozi bwo gukora igipimo cyerekana ibisubizo bishingiye ku mibare ya biliary GSSG / GSH yo guhanura ihungabana rya okiside ya hepatike.