CAS ya Dithizone: 60-10-6 80% Ifu yubururu
| Umubare wa Cataloge | XD90464 |
| izina RY'IGICURUZWA | Dithizone |
| URUBANZA | 60-10-6 |
| Inzira ya molekulari | C13H12N4S |
| Uburemere bwa molekile | 256.33 |
| Ibisobanuro birambuye | Ibidukikije |
| Amategeko agenga ibiciro | 29309098 |
Kugaragaza ibicuruzwa
| Gutakaza Kuma | ≤5% |
| Kugaragara | Ifu yubururu |
| Suzuma | 80% |
| Ibisigisigi kuri Ignition | ≤0.2% |
Mesenchymal stem selile (MSCs) byavuzwe ko ari isoko ishimishije kubyara ingirabuzimafatizo ya surrogate.Murine embryonic mesenchymal progenitor selile C3H10T1 / 2 yamenyekanye nkicyitegererezo kuri MSCs, kubera ubushobozi bwayo butandukanye.Icyari kigamijwe muri ubu bushakashatsi kwari ukureba niba selile C3H / 10T1 / 2 zifite ubushobozi bwo gutandukanya ingirabuzimafatizo zitanga insuline (IPCs).Hano, twakoze iperereza tugereranya muri vitro itandukanya imbeba MSCs na C3H10T1 / 2 muri IPC.Nyuma yuko selile zimaze gutandukana IPC, imvugo yerekana ibimenyetso bitandukanye byagaragaye na immunocytochemie, reaction transcription-polymerase urunigi (RT-PCR), umubare nyawo wa RT-PCR (qRT-PCR) hamwe na Western blotting.Ururenda rwa insuline rwasuzumwe na enzyme ifitanye isano na immunosorbent assay (ELISA).Byongeye kandi, utugingo ngengabuzima dutandukanye twatewe mu mbeba ya diyabete iterwa na streptozotocine kandi imirimo y’ibinyabuzima wer e yapimwe muri vivo.Ubu bushakashatsi buvuga ibyiciro 2 byo kubyara IPC kuva selile C3H10T1 / 2.Mugihe cyihariye cyo kwinjiza muminsi 7-8, selile C3H10T1 / 2 zakoze imibiri itatu ya spheroid (SBs) hamwe na insuline isohora, mugihe ibisekuruza bya IPC biva mumbeba MSCs byasabye igihe kirekire (ibyumweru birenga 2).Byongeye kandi, izi IPC zikomoka kuri selile C3H10T1 / 2 zatewe mu mbeba za diyabete kandi zitezimbere glucose yibanze, uburemere bwumubiri kandi zigaragaza ikizamini gisanzwe cyo kwihanganira glucose.Ubu bushakashatsi bwatanze uburyo bworoshye kandi bwizerwa muburyo bwa vitro kugirango burusheho gukora iperereza kuburyo bushingiye kuri IPC itandukanya MSCs hamwe nubuvuzi bwo gusimbuza diyabete.
