Ibicuruzwa

Imikoranire ya N7 / O6 ihuza ibikorwa bya antitumor ikora Rh (2) (OAc) (4) (H (2) O) (2) (OAc (-) = CH (3) CO (2) (-)) hamwe na ADN agace d (GpG) byagenwe bidasubirwaho na NMR spectroscopy.Mbere X-ray yerekana kristu yerekana imitwe yumutwe (HH) hamwe numutwe-umurizo (HT) wongeyeho tetraacetate ya dirhodium hamwe na 9-Ethylguanine (9-EtGH) yerekanye ikiraro kitigeze kibaho N7 / O6 guanine nucleobase izenguruka Rh Inkunga.Kubura protonation ya N7 kuri pH nkeya no kwiyongera kugaragara kwa acide ya N1-H (pK (a) hafi 5.7 ugereranije na 8.5 kuri N7 iboshye gusa ya platine), byerekanwe na pH biterwa na titre ya purine H8 (1 ) H NMR yumvikana kuri Rh (2) (OAc) (2) (9-EtG) (2) na Rh (2) (OAc) (2 -) ya nucleobase ya guanine.Indangagaciro za pK (a) zigereranijwe kuri N1-H (de) protonation, uhereye kubushakashatsi bwa pH bushingiye kuri C6 na C2 (13) C NMR resonans ya Rh (2) (OAc) (2) (9-EtG) ( 2) isomers, ihuza na t hose ikomoka kuri H8 (1) H NMR resonance titre.Kugereranya (13) C NMR resonans ya C6 na C2 kuri dirhodium yongeramo Rh (2) (OAc) (2) (9-EtG) (2) na Rh (2) (OAc) (2) [d (GpG )] hamwe na resonans ihuye na ligande zidafunze [kuri pH 7.0 kuri 9-EtGH na pH 8.0 kuri d (GpG)], yerekana ihinduka rikomeye rya Deltadelta hafi 11.0 na 6.0 ppm kuri C6 na C2;impinduka zanyuma zigaragaza ingaruka za O6 guhuza ibigo bya dirhodium hamwe no kwiyongera kwa acide ya N1-H.H8 / H8 ROE Yambukiranya impinga muri 2D ROESY NMR ya Rh (2) (OAc) (2) [d (GpG)] yerekana imitwe-imitwe ya base ya guanine.Icyongeweho cya Rh (2) (OAc) (2) inyongeramusaruro zagaragaje gusubiramo impeta ya 5'-G kuri C3'-endo (N-ubwoko), kugumana C2'-endo (S-ubwoko) ihinduka rya 3'-G isukari rin gs, hamwe no kurwanya icyerekezo kubijyanye na glycosyl.Imiterere yimiterere yabonetse kuri Rh (2) (OAc) (2)) [d (GpG)] hakoreshejwe NMR spectroscopy irasa cyane niyi cis- GpG)]] kandi bishimangira ubushakashatsi bwerekana imiterere ya molekile.