Ibicuruzwa

Spiral tube guteranya kurwanya-chromatografiya yakoreshejwe neza muri enantioseparation ya dl-tryptophan ukoresheje bovine serum albumin nkuwatoranije chiral.Sisitemu ya biphasic aqueous-aqueous solvent sisitemu 12.0% (w / w) polyethyleneglycol 8000-9.0% (w / w) dibasic potassium phosphate-0.1% ammonia-78.9% amazi yakoreshejwe nka sisitemu yo gukemura ibibazo bya chromatografiya, bovine serum albumin yiganjemo cyane mugice cyo hasi cya sisitemu y'amazi abiri.Sisitemu yo mu mazi-amazi yo mumazi yatanze enantioselectivite cyane kuri d- na l-tryptophan kuri α = 2.605 hamwe nikigereranyo cyo gukwirakwiza DD = 1.200 na DL = 0.461.Impinga ndende yabonetse kugirango enantioseparation ya 2.0mg ya dl-tryptophan na spiral tube inteko irwanya chromatografi munsi yubushyuhe bwicyumba.Byagaragaye ko 0.1% ammonia yongewe muri sisitemu yo mumazi-amazi yo mumazi yatezimbere cyane enantioseparations.Impinga idasanzwe yagutse cyane kuri l-tryptophan yagaragaye mugihe cyo kurwanya antoseparations.Kugirango dutange ibisobanuro, ibipimo byo kwimura imbaga ya d- na l-enantiomers binyuze mumirongo iri hagati yibyiciro byombi byapimwe.Byagaragaye ko l-tryptophan yerekanaga igipimo gito cyo kohereza kurusha d-tryptophan.Ibindi biganiro byasabwe kubwimpamvu zishoboka zo gutandukanya igipimo cyimibare hagati ya enantiomers.