Ibicuruzwa

Umunyamuryango mushya wumuryango wa AP2 / ERF transcription, GmERF3, yitandukanije na soya.Isesengura ryikurikiranya ryerekanye ko GmERF3 yarimo indangarugero ya AP2 / ERF ya aside amine 58 hamwe n’ibimenyetso bibiri byerekana ingufu za kirimbuzi (NLS).Yari mu itsinda rya poroteyine ya IV muri ERF (igisubizo cya Ethylene) mu buryo bworoshye nkuko byagaragajwe na motif ya N-terminal yabitswe [MCGGAI (I / L)].Kugaragaza GmERF3 byatewe no kuvura umunyu mwinshi, amapfa, aside abcisike (ABA), aside salicylic (SA), aside jasmonic (JA), Ethylene (ET), na virusi ya soya ya mozayike (SMV), mu gihe nta GmERF3 yari ihari. kwirundanya kwa mRNA mugihe cyo kuvura ubukonje.GmERF3 irashobora guhuza agasanduku ka GCC hamwe na DRE / CRT, kandi yari yibasiwe nucleus mugihe cyagaragaye mugihe gito muri selile epidermal selile.Poroteyine ya GmERF3 yahujwe na GAL4 ADN ihuza ADN kugirango itangire kwandukura genes zabanyamakuru mu musemburo.Imvugo ya ectopique yerekana gene ya GmERF3 mu bimera by’itabi byatewe na PR ge nes kandi byongereye imbaraga zo kurwanya kwandura virusi ya Ralstonia solanacearum, Alternaria alternata, na virusi ya mosaic virusi (TMV), kandi bihanganira umunyu mwinshi hamwe n’ingutu ziterwa no kubura umwuma.Byongeye kandi, gukabya gukabije kwa GmERF3 mu itabi rya transgenji byatumye habaho urugero rwinshi rwa porine na karubone nziza cyane ugereranije n’ibiti byo mu gasozi mu bihe by’amapfa.Ibisubizo muri rusange byerekanaga ko GmERF3 nkibintu byandikirwa AP2 / ERF bishobora kugira uruhare runini mugusubiza ibibazo bya biotic na abiotic mubihingwa.