Ibicuruzwa

ସିଟିGukoresha Lipid ya CCTα bituma ihindurwa mu ibahasha ya kirimbuzi no kwagura urusobe rw’ibisasu byitwa nucleoplasmic reticulum (NR) hakoreshejwe uburyo bujyanye no guhindura ibintu.Intwaro za kirimbuzi nazo zirakenewe kugirango ituze no gukwirakwira kwa NR, ariko niba iyi miterere idasanzwe, cyangwa lamina ya kirimbuzi muri rusange, irakenewe kugirango synthesis ya PC ntizwi.Kugira ngo dusuzume uyu mubano, lamina ya kirimbuzi yagabanutse na RNAi cyangwa ihungabanywa no kwerekana syndrome ya Hutchinson-Gilford progeria (HGPS) mutant lamin A (progerin), maze hasesengurwa ingaruka kuri CCTα na choline metabolism.gucecekesha siRNA yifashishijwe gucecekesha lamin A / C cyangwa lamin B1 mu tugari twa CHO kugirango igabanye NR nta ngaruka byagize kuri synthesis ya PC, mugihe gukubita kabiri bitabujije inzira inzira.Kwemeza uruhare rwe ruto muri synthesis ya PC, 10% gusa bya choline / ethanolamine fosifotransferase yagaragaye cyane muri NR.Muri selile CHO, CCTα yari nucleoplasmic kandi ifatanya na GFP-progerine mubice bya kirimbuzi no gutera;icyakora, fibroblast ya HGPS yerekanye ikwirakwizwa ridasanzwe rya CCTα muri cytoplazme no mu ibahasha ya kirimbuzi yaherekejwe no kugabanuka inshuro 2 muri synthesis ya PC.Nuburyo bwahinduwe bwaho, ubushakashatsi bwanditse kuri choline bwerekanye ko ibikorwa bya CCT ntacyo byagize, kandi kubuza synthesis ya PC byagaragaye ko byagabanije ibikorwa byabatwara choline iterwa na hemicholinium.Twanzuye ko CCTα na lamine bifatanya cyane cyane gushinga NR, ariko imiterere rusange y ibahasha ya kirimbuzi igira ingaruka nkeya kubikorwa bya CCT hamwe na synthesis ya PC.