Chymotrypsinogen A CAS: 9035-75-0 Ifu yera ya kristaline
| Umubare wa Cataloge | XD90376 |
| izina RY'IGICURUZWA | Chymotrypsinogen A. |
| URUBANZA | 9035-75-0 |
| Inzira ya molekulari | C47H58FeN4O4S2 |
| Uburemere bwa molekile | 862.96 |
| Ibisobanuro birambuye | -20 ° C. |
Kugaragaza ibicuruzwa
| Suzuma | 99% |
| Kugaragara | Ifu yera ya kristaline |
Kwinjiza poroteyine kumasemburo akomeye-akunze kuboneka muburyo bwogutezimbere kugirango habeho kwibumbira hamwe kandi binini, bigabanywa, kandi bigaragara, ariko ibyiciro byingenzi muriki gikorwa biragoye kubigenzura muburyo butaziguye.Igiteranyo cyahujwe na adsorption kumazi-silicon oxyde (SiOx), bisa nkibirahure byikirahure, byaranzwe nkigikorwa cya pH nimbaraga za ionic kuri alpha-chymotrypsinogen (aCgn) no kuri antibody ya monoclonal (IgG1).Ingirabuzimafatizo yatumaga neutron igaragarira kuri poroteyine yamamajwe kugirango isukure hejuru ya SiOx, kimwe na nyuma yo gukaraba "gukaraba".Igiteranyo cyakuwe mubisubizo nyuma yo "kwoza" buhoro buhoro hejuru yaranzwe no gukwirakwiza neutron, microscopi, na fluorescence spectroscopy.Molekile ya IgG1 yerekeje cyane cyane "iringaniye" hejuru ya SiOx, hamwe na poroteyine yambere ya poroteyine yamanutse ku rugero ruto, mu gihe ikwirakwizwa rya diffuse ryarogejwe byoroshye.molekile ya aCgn yarwanyaga desorption mugihe yabaga yiteguye gukingurwa kuri interineti, ariko ubundi ikurwaho byoroshye.Ku bihe aho guhuza gukomeye kwabaye, poroteyine yakoze desorb yari imvange ya monomer hamwe na HMW igiteranyo gito (kuri aCgn) cyangwa uduce duto duto (kuri IgG1).Impinduka muri adsorption na / cyangwa gufungura hamwe na pH byerekanaga ko imikoranire ya electrostatike yari ingenzi mubihe byose.
