Ibicuruzwa

Gukurikirana imiti ya farumasi usibye imiti ya farumasi nimwe mungamba zo gutandukanya mycophenolate mofetil ivura.Icyari kigamijwe muri ubu bushakashatsi kwari ugutezimbere uburyo bworoshye bwa chromatografiya-tandem mass spectrometrie (LC-MS / MS) uburyo bwo gusuzuma imiti ya farumasi na farumasi ya acide mycophenolike (MPA).Ihuriro rya acide mycophenolique glucuronide (MPAG), acide mycophenolike acyl-glucuronide, kimwe na MPA na MPAG idafitanye isano, harabazwe, kandi inosine-5'-monophosphate dehydrogenase yabazwe hifashishijwe gupima ubukana bwa xanthosine-5'-monophosphate (XMP) ) na adenosine adenosine-5'-monophosphate nyuma yubushakashatsi bwamaraso ya periferique mononuclear selile (PBMC) lysates.Inkingi idafite ibyuma bya Mastro (TM) hamwe nuburyo bubiri bwa gradient byakoreshejwe mugutezimbere igipimo cya XMP kugeza 0.1 μM.Mu rwego rwo kwibanda ku mavuriro ya MPA, umurongo uhuza umurongo wa kalibrasi, hagati yumunsi no hagati yumunsi nukuri kandi byuzuye byujuje ibyerekeranye n’ibimonyo byo muri Amerika bishinzwe ibiryo n'ibiyobyabwenge.Ubwinshi bwa MPA mu barwayi ba hematopoietic stem selile transplant (HSCT) bugenwa na enzyme assay hamwe nubu LC-MS / MS bwerekanye isano nziza (r (2) = 0,95, p <0.001).Muri ubu bushakashatsi, turatanga raporo yuburyo bworoshye kandi bwemewe bwa LC-MS / MS kugirango dusuzume imiti ya farumasi na farumasi ya MPA, zumva neza bihagije gusuzuma umubare muto wa lysates ya PBMC yakusanyijwe nyuma gato ya HSCT.