Ibicuruzwa

Ikirangantego kitarimo electrochemiluminescence (ECL) immunosensor kugirango ultrasensitive kandi yihuse yerekane bacterium zo mu nyanja Vibrio parahaemolyticus (VP) mumazi yinyanja nibiryo byo mu nyanja byakozwe hifashishijwe okiside ya graphene ikora cyane, yateguwe na N- (4-aminobutyl) -N -ethylisoluminol (ABEI) na antibody ya VP (anti-VP) icyarimwe yimuwe hejuru ya oxyde ya magnetiki graphene (nanoFe3O4 @ GO).ABEI na anti-VP bakoze nka electrochemiluminophore nigikoresho cyo gufata kuri VP.Imyitwarire myiza nuburyo bubiri bwa nanoFe3O4 @ GO yashoboje ABEI yose, yimuwe kuri GO, ikora amashanyarazi bityo ikanonosora ibyiyumvo byo kumenya.Mugihe gikwiye, ubukana bwa ECL bwaragabanutse hamwe no kwiyongera kwa logarithmic ya VP murwego rwa 10-10 (8) CFU / mL, hamwe na 5CFU / mL yo kumenya amazi yinyanja na 5CFU / g kubiryo byo mu nyanja.Iyi immunosensor ya ECL yerekanye umwihariko, ituze hamwe niyororoka kugirango tumenye VP.Byongeye kandi, immunosensor ya ECL yakoreshejwe neza kugirango hamenyekane ubukana bwa VP mu mazi yo mu nyanja n’ibiryo byo mu nyanja byihuse, hamwe na 94.4-112.0% na RSD 4.1-11.7%.Kubwibyo, immunosensor yateye imbere yerekana ibyiringiro byinshi byo gushyira mubikorwa.