Ibicuruzwa

Kurenga 70% ya glioma yo mu rwego rwo hasi itwara ihinduka rya heterozygous R132H muri gene code ya isocitrate dehydrogenase 1 (IDH1).Ibi bitanga enzyme nubushobozi bushya bwo guhindura α-ketoglutarate kuri 2-hydroxyglutarate, amaherezo biganisha kuri tumorigenez.Inkomoko nyamukuru y’umusaruro wa 2-hydroxyglutarate ni glutamine, iyo, muri kanseri, nayo ikaba isoko ya acide tricarboxylic (TCA) anaplerose.Ubundi buryo buturuka kuri anaplerose ni pyruvate flux ikoresheje pyruvate carboxylase (PC), ninzira isanzwe muri astrocytes zisanzwe.Intego yubu bushakashatsi yari iyo kumenya niba PC ikora nkisoko ya TCA anaplerose mungirangingo ya IDH1 aho glutamine ikoreshwa mu musaruro wa hydroxyglutarate 2.Amazi ya pyruvate akoresheje PC kandi akoresheje pyruvate dehydrogenase (PDH) yagenwe hakoreshejwe magnetiki resonance spectroscopy kugirango asuzume ikirango cya [2-¹³C] glucose ikomoka kuri ¹³C-labe yayoboye glutamate na glutamine.Ibikorwa byerekana, RT-PCR na blotting yuburengerazuba byakoreshejwe mugusuzuma imvugo nigikorwa cyimisemburo ifitanye isano.Isesengura rya Kanseri Genome Atlas (TCGA) ryasesenguwe kugira ngo hamenyekane imvugo ya enzymes mu ngero za glioma ya muntu. Ugereranije n'ingirabuzimafatizo zo mu gasozi, selile IDH1 ya mutant yongereye cyane ibice biva muri PC.Ibi byari bifitanye isano no kwiyongera cyane mubikorwa bya PC no kwerekana.Mugihe kimwe, ibikorwa bya PDH byagabanutse cyane, birashoboka ko byahujwe no kwiyongera cyane kubuza PDH fosifora na PDH kinase 3. Bihuye nubushakashatsi bwakozwe mu tugari, isesengura ryamakuru ya TCGA ryerekanye ko kwiyongera kwa PC kugaragara muri mutant IDH yerekana ingero za glioma zabantu ugereranije n’ishyamba- andika IDH.Ibisubizo byacu byerekana ko impinduka muri PC na PDH zishobora kuba igice cyingenzi cyo guhuza ingirabuzimafatizo na IDH1 kandi bishobora kuba intego yo kuvura.