4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE URUBANZA: 10357-27-4 Ifu Yera Yera 98%
| Umubare wa Cataloge | XD90011 |
| izina RY'IGICURUZWA | 4-Nitrophenyl-alpha-D-mannopyranoside |
| URUBANZA | 10357-27-4 |
| Inzira ya molekulari | C12H15NO8 |
| Uburemere bwa molekile | 30301.25 |
| Ibisobanuro birambuye | -2Kuri -8° C. |
| Amategeko agenga ibiciro | 29400000 |
Kugaragaza ibicuruzwa
| Amazi | <5% Karl Fische |
| Gukemura | 1% muri DMF irasobanutse kandi idafite ibara |
| Isuku | Ubuntu 4-Nitropenol <200ppm |
| HPLC | > 98% |
| Kugaragara | Ifu yera |
Ubushishozi bwibikoresho bya Ca2 + -bishingiye kumuryango wa alpha-mannosidase mumyanya yumuntu.
Bagiteri zo mu bwoko bwa colonike, zigaragazwa na Bacteroides thetaiotaomicron, zigira uruhare runini mu kubungabunga ubuzima bw’abantu hifashishijwe imiryango minini ya hydrolase ya glycoside (GHs) kugira ngo ikoreshe polysaccharide y’imirire ndetse na glycans nk'intungamubiri.Kwiyongera kwumuryango wa GH bigaragazwa nimiryango 23 GH92 glycosidase yashizweho na genome ya B. thetaiotaomicron.Hano twerekana ko alfa-mannosidase ikora ikoresheje uburyo bumwe bwo kwimura abantu kugirango bakoreshe N-glycans.Imiterere-yuburyo butatu ya mannosidase ebyiri za GH92 isobanura umuryango wa poroteyine zibiri aho ikigo cya catalitiki giherereye kuri interineti, gitanga aside (glutamate) nubufasha bwibanze (aspartate) kuri hydrolysis muri Ca (2 +) - uburyo bushingiye.Imiterere-yuburyo butatu ya GH92s murwego hamwe na inhibitor itanga ubushishozi muburyo bwihariye, uburyo hamwe ninzira ihuza catalizike.Ca (2+) igira uruhare runini mu gufasha kugoreka mannoside kure yubutaka bwayo (4) C (1) intebe ihinduka yerekeza kuri leta yinzibacyuho. （Bibliografiya: Nat.Chem.Biol.6, 125-32, (2010）
Imbere yimbere ya chromatografiya ya ovalbumin glycoasparagines kumurongo wa A-sepharose.Kwiga kwinshi kubijyanye no guhuza umwihariko w'inyigisho.
Imikoranire ya Sepharose 4B-immobilized concanavalin A (ConA) na glycoasparagine 10 ikomoka kuri ovalbumin yakozweho ubushakashatsi ku bwinshi na chromatografi yimbere.Muri ubu buryo, igisubizo cya karubidiyatire gikoreshwa ubudahwema kumurongo wa ConA-Sepharose kandi kudindiza imbere ya elution bipimwa nkibipimo byimbaraga zimikoranire.Gutandukana guhoraho (Kd) kuri buri sakaride hamwe na ConA birashobora kugenwa.Isesengura ryihuza rya p-nitrophenyl-alpha, D-mannoside ryerekanye ko imiterere ihuza ConA idahinduka cyane nyuma yo kwimuka kuri Sepharose 4B.Buri kimwe muri ovalbumin glycoasparagines cyanditswemo tritium nuburyo bwo kugabanya methylation yo gusesengura.Kugereranya indangagaciro za Kd zabonywe zerekanaga ko guhuza ConA bigenda bitandukana cyane nuburyo butandukanye bwimiterere itandukanye ya glycosyl.Ibisubizo byerekana ko ConA izi imiterere yihariye ya glycosyl, Umuntu alpha 1-6 (Umuntu alpha 1-3) Umuntu, aho byibuze itsinda rimwe rya hydroxyl kumwanya C-3 kumwanya wa C-6 uhujwe na mannose.
