4-Nitrophenyl-alpha-D-galactopyranoside CAS: 7493-95-0 99%
| Umubare wa Cataloge | XD900018 |
| izina RY'IGICURUZWA | 4-Nitrophenyl-alpha-D-galactopyranoside |
| URUBANZA | 7493-95-0 |
| Inzira ya molekulari | C12H15NO8 |
| Uburemere bwa molekile | 301.25 |
| Ibisobanuro birambuye | -15 kugeza kuri 20 ° C. |
| Amategeko agenga ibiciro | 29400000 |
Kugaragaza ibicuruzwa
| Kugaragara | Umweru kuri Orange kugeza icyatsi kibisi kuri kristu |
| Suzuma | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) ni insimburangingo ya 4-nitrophenyl (pNP) glycopyranoside kugirango hamenyekane ibikorwa bya α-galactosidase.Umubare wa pNP wasohotse wariyongereye cyane mugihe 4-Nitrophenyl α-D-galactopyranoside yakoreshejwe nka substrate.
Ukoresheje4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenylα-D-galactopyranosideissignificificificantigigherthanthatfromtheotherpNP-glycopyranosidesatpH4.0 ahantu hose henyl (pNP) glycopyranosidesasartificialsubstratesshowsthatyieldinliberatedgalactoseresiduesfrom4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, iethepHlevelwhereyieldthresholdisdecreasedatmost.
Substrate ya α-D-galactosidase (α-D-Galactosidase).Ihambire kuri E. coli lactose itwara protein.Hagati ya α-D-galactose.
Hashyizweho uburyo bwihuse bwo gusuzuma ibikorwa bya mikorobe yica mikorobe irwanya umusemburo n ibihumyo.Isuzuma rishingiye ku irekurwa rya enzyme yo mu nda, maltase (alpha-glucosidase).Igikorwa cya maltase cyasohotse cyapimwe muburyo bwo gukora p-nitropenol ikomoka kuri p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Igikorwa cya lytike yibintu bitandukanye bivura mikorobe byapimwe kurwanya selile cyangwa imisemburo imera yibihumyo.Igikorwa cyo kurwanya umusemburo gishobora kugaragara mu minota 20 yubushyuhe kuri dogere 30 C kurwanya Saccharomyces cerevisiae, Candida albicans, na Cryptococcus neoformans.Igikorwa cya Lytic anti-fungal cyagaragaye nyuma ya 2 h yubushakashatsi kuri dogere 30 C kurwanya spore zimera za Aspergillus niger na Botrytis cinerea.Ingirabuzimafatizo zose zaba umusemburo cyangwa ibihumyo ntabwo hydrolyze PNPG ihagije muri 3 h kuri dogere 30 C kugirango itange amabara agaragara.Ibikorwa bya Litike ya enzymes (urugero, Lyticase), antibiotike (urugero, Amphotericine B), hamwe na antibiyotike itanga antibiyotike ya bagiteri byagaragaye hakoreshejwe ubushakashatsi.Kurwanya umusemburo byahinduwe muburyo bwa microtiter 96-nziza.Ubushakashatsi bwombi bwatanze uburyo bwihuse, bworoshye, kandi bwororoka bwerekana ibikorwa bya lytic anti-umusemburo no kurwanya fungal.
