4-Amino-3-hydrazino-1,2,4-triazol-5-thiol Cas: 28836-03-5 99% Umuhondo kugeza icyatsi kibisi
| Umubare wa Cataloge | XD90146 |
| izina RY'IGICURUZWA | 4-Amino-3-hydrazino-1,2,4-triazol-5-thiol |
| URUBANZA | 28836-03-5 |
| Inzira ya molekulari | C16H13NO3S · NH3 |
| Uburemere bwa molekile | 316.37 |
| Ibisobanuro birambuye | Ibidukikije |
| Amategeko agenga ibiciro | 2923900090 |
Kugaragaza ibicuruzwa
| Kugaragara | Umuhondo kugeza icyatsi gikomeye |
| Assay | ≥ 99% |
| Ingingo yo gushonga | 237 ° C (Ukuboza) (lit.) |
| Gukemura | NaOH: gukemura1 N. |
| Amazi ashonga | Gushonga mumazi, 1N NaOH, na methanol. |
1.Twize ingaruka zibuza isorhamnetin kuri ibihumyo tyrosinase muguhagarika kinetics no kwigana kubara.Isorhamnetin yahagaritse tyrosinase muburyo buvanze muburyo bwa Ki = 0.235 ± 0.013 mM.Ibipimo byimbere na 1-anilinonaphthalene-8-sulfonate (ANS) -guhuza fluorescence byerekanaga ko isorhamnetin itigeze itera impinduka zikomeye mumiterere ya kaminuza ya tyrosinase.Kugirango ubone ubushishozi mubikorwa byo kudakora, kinetics yabazwe hakoreshejwe ibipimo byigihe-intera hamwe na substrate reaction.Ibisubizo byerekanaga ko kudakora byatewe na isorhamnetin byari igisubizo cya mbere hamwe na biphasic nzira.Kugirango turusheho gushishoza, twiganye docking hagati ya tyrosinase na isorhamnetin.Kwigana byagenze neza (guhuza ingufu kuri Dock6.3: -32.58 kcal / mol, kuri AutoDock4.2: -5.66 kcal / mol, no kuri Fred2.2: -48.86 kcal / mol), byerekana ko isorhamnetin ikorana nibisigisigi byinshi, nkibi nka HIS244 na MET280.Izi ngamba zo guhuza tyrosinase zifatika zifatanije na kinetics zishingiye ku gihimba cya flavanone zishobora kuba ingirakamaro mugusuzuma ibishobora kuba byangiza tyrosinase.
2.Umuhanda ugenda uhinduka wa conalbumin (CA), glycoproteine ya monomeric iva mu magi y’inkoko yera, yakozweho iperereza hakoreshejwe CD ya spekitroscopi ya kure na hafi ya UV, imyuka yoherezwa mu kirere, fluorescence probe 1-anilino-8-napthalene sulfonate (ANS) n'umucyo ukwirakwiza (DLS).Twitegereje impinduka zishingiye kuri pH muburyo bwa kabiri na kaminuza ya CA.Ifite kavukire-isa α-ihindagurika yuburyo bwa kabiri kuri pH 4.0 ariko igihombo kuri pH 3.0.CA yabayeho gusa nka globule yabanje gushonga hamwe na globule leta yashongeshejwe mugisubizo kuri pH 4.0 na pH 3.0.Ingaruka ya pH kumihindagurikire hamwe nubushyuhe bwa CA yerekeza kubushyuhe bwayo kuri pH itabogamye.Ibisubizo bya DLS byerekana ko leta ya MG yabayeho nkuburyo bworoshye mubisubizo byamazi hamwe na hydrodynamic radii ya 4.7 nm.Kuzimya fluorescence ya tryptophan na acrylamide byongeye kwemeza kwegeranya leta yo hagati, igice cyagaragaye, hagati yigihugu kavukire kandi kidafunguwe.
