Ibicuruzwa

Chemifluorescence: Molekulari luminol ni molekile ya fluorescent ya molekile ishobora guhinduka aside aside aminophthalic ishimishije imbere ya molekile ya hydrogen peroxide, itanga fluorescence ikomeye.Hydrogene peroxide nigicuruzwa cya biooxidative reaction nyinshi, biroroshye rero guhuza izo biooxidative reaction na Photodetection mugutangiza luminol.Kurugero, glucose oxydease / catalase probe irashobora kumenya ubunini bwa hydrogen peroxide cyangwa glucose murugero, kandi igihe cyo gusubiza ni 0.5s gusa (uburyo bwa dinamike).Gukomatanya ibintu bya chemiluminescent hamwe nubudahangarwa bw'umubiri, no kwerekana ubwinshi bwibigize ubudahangarwa bwamenyekanye hakoreshejwe urumuri, byitwa chemiluminescent immunoassay.Mu 1976, Shmeder yatanze imiti ya chemiluminescence immunoassay, akoresheje luminol-H202 hamwe n’ibiyikomokaho ABEI nka sisitemu yo kwerekana reaction.Kugeza ubu, luminol n'ibiyikomokaho nibimenyetso bikoreshwa cyane muri chemiluminescence immunoassay ikoreshwa.Mugihe cya alkaline, itangizwa na microperoxidase, umubare munini wa fotone urashobora kurekurwa.Luminol irashobora gukoreshwa kuri WesternBlot ya HRP yanditswemo antibodies hamwe na acide nucleic aside ivanga na HRP yanditseho probe.Irakoreshwa kandi mugushakisha ubugizi bwa nabi bwa kijyambere.Uruvange rushobora gukoreshwa nkikimenyetso cya chemiluminescent rugomba kuba rwujuje ibi bikurikira: umusaruro wa kwantum wa luminescence ni mwinshi;imiterere ya fiziki-chimique ihuye na sisitemu iri kwigwa;reaction ya luminescent nigisubizo cya okiside reaction ya luminescent;murwego rwo kwibandaho rukoreshwa Imbere idafite uburozi kubinyabuzima.Ubwoko butandukanye bwa chemiluminescence reagent ikoreshwa cyane: estride acridine, tracer ya chemiluminescence ikoreshwa cyane, ni uruganda rwa tricyclicique, rworoshye okiside, kandi reaction ya okiside ntisaba catalizator, kandi irekura fotone kuri 430nm;Luminol na isoluminol n'ibiyikomokaho ni imiti ya chemiluminescent ikuze cyane.Nko mu 1964, Mugihe byavuzwe kuri chemiluminescence, luminol, isoluminol nibibikomokaho byakoreshejwe muri chemiluminescence.Immunoassays yageze kubisubizo byiza.Kugaragaza Luminescence: uburebure bwiza bwa fluorescence ni 400nm (gutahura chemiluminescence muri 60mmK2S2O8, 100mmK2CO3, pH11.5 igisubizo)

Chemiluminescence: Nuburyo bworoshye bwo gusuzuma, chemiluminescence ikoreshwa cyane mugutahura radicals yubusa na metabolite reaction ikorwa mumisemburo, selile nibinyabuzima.Umucyo utangwa nibicuruzwa bya okiside na metabolite birashobora gukoreshwa hamwe na fotometero zitandukanye.gutahura.Chemiluminescence ikoreshwa cyane mugupima no gukora ubushakashatsi kuri antioxydants, nka polifenol, polysaccharide, flavonoide, na anthraquinone, kubera sensibilité yayo, yihuta, imikorere yoroshye nigiciro gito.Sisitemu ikoreshwa cyane ya chemiluminescence muguhitamo anion ya superoxide harimo xanthine oxydease-luminol, pyrogallol-luminol na dimethyl sulfoxide-luminol.Iyambere ni sisitemu ya enzymatique, naho iyanyuma ni Sisitemu itari enzymatique.Sisitemu ya chemiluminescence kugirango hamenyekane radicals ya hydroxyl harimo cyane cyane umuringa wa sulfate-umusemburo (cyangwa ingirabuzimafatizo) sisitemu ya peroxide 5 ya chemiluminescence, ferrous sulfate-luminol-hydrogen peroxide na ferrous sulfate-luminol, byose birimo reaction ya Fenton ya classique ya hydroxyl radicals, hanyuma yibasira umukozi wa luminescent kubyara chemiluminescence, ishobora gukoreshwa mugupima ikurwaho ryibikuramo The ibikorwa bya ogisijeni ikora neza.Uburyo bw'igerageza: koresha sisitemu ya pyrogallol-luminol chemiluminescence: luminol ikozwe mumashanyarazi ya 0.05mol / L hamwe na 0.05mol / L NaOH igisubizo, kibikwa ahantu hijimye, n'amazi yatoboye kabiri mbere yo gukoresha Dilute kugeza 1mmol / L, koresha 1mmol / L.0.05mol / L pH10.Mugihe cyo gupima, shyiramo 10.0 μL ingero zubushakashatsi butandukanye (0, 0.08, 0.4, 2 na 10 mg / mL) muri selire ya luminescent (hamwe na bffer sample yo kugenzura), hanyuma utere 6.25 × 10-4mol / L pyrogallol 0.05 Hanyuma, 0,94 mL yuruvange rwa luminol na aside ya karubone yongeweho kugirango itangire reaction (30 ° C), ubukana bwa luminescence bwabazwe intera ya s 2, hanyuma hapimwa ubukana bwa luminescence kuri 300 s.Inyuma ya luminescence ubukana bwari luminescence mugihe pyrogallol itongeyeho.agaciro.Mubyongeyeho, hariho chemiluminescence, fluorescence spectroscopy na sisitemu ya NBS-dichlorofluorescein chemiluminescence kugirango hamenyekane fluorescence chemiluminescence, nibindi, inyungu zabo zikomeye nukwiyumvamo cyane.

Ibikoresho bya Shimi: Ifu ya kristaline yumuhondo.Byoroshye gushonga muri lye, gushonga muri acide ya dilute, hafi yo kudashonga mumazi, no kudashonga muri alcool.Ibisubizo bidafite aho bibogamiye cyangwa acide nkeya byerekana florescence ikomeye yubururu iyo ihuye nurumuri ultraviolet.Gushonga ingingo 329-332 ℃

Gukoresha: Nka analyse yimiti reagent nicyerekana.Koresha Chemiluminescence isesengura ryerekana reagent (nko kumenya ibyuma cyangwa amaraso)

Gukoresha: Kubisesengura rya chemiluminescence, nka: ibyuma byuma, amaraso na glucocorticoide

Ikoreshwa: Ikizamini cya Luminescence: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; nyuma yo kongeramo H2O2) chemiluminescence reagent nicyerekana, bikunze gukoreshwa mubisesengura rya chemiluminescence, nka cations cuma, ubudahangarwa bwamaraso, nibindi.