Ibicuruzwa

Twabanje guhura nuburyo bwo kugenzura aho dihydrotestosterone (DHT) yagize ingaruka mbi zo guhagarika imisemburo ya parathiyide yatewe na poroteyine (PTHrP) ikoresheje gene ya estrogene (ER) α, ariko ntabwo yakira andorogene (AR), muri kanseri y'ibere MCF-7 .Hano, twasesenguye niba imikoranire nkiyi ya aberrant ligand-nuclear reseptor (NR) igaragara muri kanseri ya prostate LNCaP.Ubwa mbere, twemeje ko selile LNCaP yerekanaga AR nyinshi murwego rwa ERα / β cyangwa reseptor ya progesterone.Kurwanya PTHrP no gukora genoside yihariye ya prostate byagaragaye nyuma yubutegetsi bwigenga bwa 17β-estradiol (E2), DHT, cyangwa R5020.Mu buryo buhuye n’igitekerezo kivuga ko LNCaP AR yatakaje umwihariko wa ligand kubera ihinduka ry’imihindagurikire (Thr-Ala877), ubushakashatsi bwakozwe na siRNA bwibasiye NR bireba bwerekanye ko AR yihariye uruhare rw’umuhuza w’imisemburo isangiwe na hormone, ibyo bikaba byari bifitanye isano buri gihe. hamwe na translocatio ya kirimbuzi n yiyi mutant AR.Isesengura rya Microarray ryerekeye amabwiriza ya gene ryakozwe na DHT, E2, cyangwa R5020 ryagaragaje ko kimwe cya kabiri cya gen zigabanuka munsi ya AR (Thr-Ala877) zuzuye mu ngirabuzimafatizo za LNCaP.Mubyifuzo byumwihariko, twabonye ko AR (ubwoko bwishyamba [wt]) na AR (Thr-Ala877) bashinzwe kimwe mubikorwa bya E2-AR.Ubushakashatsi bwa microscopi ya Fluorescence bwerekanye ko EGFP-AR (wt) na EGFP-AR (Thr-Ala877) byombi byashyizwe muri nucleus nyuma yo kuvurwa E2 cyangwa DHT.Byongeye kandi, umunyamakuru avuga ko izindi selile zimwe na zimwe za kanseri zagaragaje aberrant E2-AR (wt) zerekana ibimenyetso bisa nkibyo mu tugari twa LNCaP.Hano turerekana ko hariho imikoranire ifitanye isano na wt AR na E2 muri selile zimwe na zimwe zitera kanseri.